An integrated encyclopedia of DNA elements in the human genome

nfkbcell

An integrated encyclopedia of DNA elements in the human genome.

 

The human genome encodes the blueprint of life, but the function of the vast majority of its nearly three billion bases is unknown. The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped regions of transcription, transcription factor association, chromatin structure and histone modification.

 

These data enabled us to assign biochemical functions for 80% of the genome, in particular outside of the well-studied protein-coding regions. Many discovered candidate regulatory elements are physically associated with one another and with expressed genes, providing new insights into the mechanisms of gene regulation.

 

The newly identified elements also show a statistical correspondence to sequence variants linked to human disease, and can thereby guide interpretation of this variation.

 

Overall, the project provides new insights into the organization and regulation of our genes and genome, and is an expansive resource of functional annotations for biomedical research.

nfkbcell
nfkbcell

Genomic DNA Kit

20-abx098076
  • EUR 439.00
  • EUR 244.00

CRISPR Genomic Cleavage Detection Kit

G932
EUR 205

CRISPR Genomic Cleavage Detection Kit

K1250-25
EUR 403

5-min. Genomic DNA Prep for PCR

P930-100 Ask for price

5-min. Genomic DNA Prep for PCR

P930-5 Ask for price

5-min. Genomic DNA Prep for PCR

P930-500 Ask for price

Human Brain Genomic DNA

X11001 Ask for price
Description: kits suitable for this type of research

Plant Genomic DNA Kit

20-abx098077
  • EUR 411.00
  • EUR 230.00

Bacteria Genomic DNA Kit

20-abx098080
  • EUR 105.00
  • EUR 244.00

Micro Genomic DNA Kit

20-abx098242
  • EUR 105.00
  • EUR 328.00

Blood Genomic DNA Kit

abx098868-50rxns
EUR 272

Plant Genomic DNA Kit

abx294004-50preps
EUR 398

Animal Genomic DNA Kit

abx294005-100preps
EUR 523

Animal Genomic DNA Kit

abx294005-50preps
EUR 398

Genomic DNA Isolation Kit

K2118-50
EUR 321

Genomic DNA Isolation Kit

55R-1362
EUR 385
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA - Human Male

D1234999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Human Female

D1234999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Male

D1334999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Mouse Female

D1334999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Male

D1434999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rat Female

D1434999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Male

D1734999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Dog Female

D1734999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Male

D1834999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rabbit Female

D1834999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Male

D1934999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Porcine Female

D1934999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Male

D1B34999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Bovine Female

D1B34999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Male

D1C34999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Chicken Female

D1C34999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Male

D1H34999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Hamster Female

D1H34999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Male

D1O34999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Horse Female

D1O34999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA Extraction Kit

K5016005
EUR 329

Soil Genomic DNA Kit

K1411-250
EUR 561

Soil Genomic DNA Kit

K1411-50
EUR 316

Insect Genomic DNA Kit

K1412-250
EUR 561

Insect Genomic DNA Kit

K1412-50
EUR 316

Mollusc Genomic DNA Kit

K1413-250
EUR 561

Mollusc Genomic DNA Kit

K1413-50
EUR 316

Yeast Genomic DNA Kit

K1414-250
EUR 561

Yeast Genomic DNA Kit

K1414-50
EUR 316

Fungal Genomic DNA Kit

K1415-250
EUR 561

Fungal Genomic DNA Kit

K1415-50
EUR 316

Genomic DNA Isolation Kit

K281-50
EUR 272

Shigella Sonnei Protein

abx061489-01ml
EUR 551

DNA Methylation Detection Kit

K5082100
EUR 344

Genomic DNA Extraction and Purification

EP10012
EUR 505

Genomic DNA Extraction KitAnimal Tissue

FYG102-100P Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-300P Ask for price

Genomic DNA Extraction KitAnimal Tissue

FYG102-50P Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-10P Ask for price

Genomic DNA Extraction Maxi KitPlant

FYG104-25P Ask for price

ISOLATE II Genomic DNA Kit

BIO-52065 Ask for price

ISOLATE II Genomic DNA Kit

BIO-52066 Ask for price

ISOLATE II Genomic DNA Kit

BIO-52067 Ask for price

Plant Genomic DNA extraction buffer

20-abx098075
  • EUR 286.00
  • EUR 105.00

Marine Animal Genomic DNA Kit

20-abx098079
  • EUR 105.00
  • EUR 244.00

FFPE Tissue Genomic DNA Kit

abx098866-50rxns
EUR 272

Zenoquick Genomic DNA Purification Kit

Z2003-200
EUR 631

Zenoprep Genomic DNA Purification Kit

Z2004-400
EUR 550

AccuRT Genomic DNA Removal Kit

G488
EUR 82

Genomic DNA - Cynomolgus Monkey Male

D1534999-Cy-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Cynomolgus Monkey Female

D1534999-Cy-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Male

D1534999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Rhesus Monkey Female

D1534999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Male

D1G34999-G01
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA - Guinea Pig Female

D1G34999-G02
EUR 188
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Bacterial Genomic DNA Isolation Kit

K309-100
EUR 376

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Mouse antibody for Shigella sonnei form 1^ LPS

945
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Shigella sonnei form 1^ LPS for WB, ELISA.

Mouse antibody for Shigella sonnei form 1^ LPS

946
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Shigella sonnei form 1^ LPS for WB, ELISA.

Mouse antibody for Shigella sonnei form 1^ LPS

947
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Shigella sonnei form 1^ LPS for WB, ELISA.

Rapid Fungal Genomic DNA Isolation Kit

FT71415
EUR 76.64

Genomic DNA Extraction Mini KitPlant Tissue

FYG103-100P Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-100P Ask for price

Easy3-Plant Genomic DNA Isolation Kit

FYG503-500P Ask for price

Fully CpG Methylated Human Genomic DNA

X10000 Ask for price
Description: The best epigenetics products

Rapid Bacteria Genomic DNA Isolation Kit

BS8225
EUR 76.64

Rapid Yeast Genomic DNA Extraction Kit

BS8227
EUR 76.64

Rapid Blood Genomic DNA Extraction Kit

BT4782
EUR 76.64

Rapid Animal Genomic DNA Extraction Kit

AT4780
EUR 69.03

 

Studies on transformation of Escherichia coli with plasmids.

 

Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth in medium containing elevated levels of Mg2+, and incubation of the cells at 0 degrees C in a solution of Mn2+, Ca2+, Rb+ or K+, dimethyl sulfoxide, dithiothreitol, and hexamine cobalt (III). Transformation efficiency declines linearly with increasing plasmid size.

 

Relaxed and supercoiled plasmids transform with similar probabilities. Non-transforming DNAs compete consistent with mass. No significant variation is observed between competing DNAs of different source, complexity, length or form.

 

Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmids.

 

Transformation of intact yeast cells treated with alkali cations.

ntact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl.

 

The optimum incubation time was 1 h, and the optimum cell concentration was 5 x 10(7) cells per ml. The optimum concentration of Cs+ was 1.0 M. Transformation efficiency increased with increasing concentrations of plasmid DNA.

 

Polyethylene glycol was absolutely required. Heat pulse and various polyamines or basic proteins stimulated the uptake of plasmid DNA. Besides circular DNA, linear plasmid DNA was also taken up by Cs+-treated yeast cells, although the uptake efficiency was considerably reduced.

 

The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.

Improved tools for biological sequence comparison.

We have developed three computer programs for comparisons of protein and DNA sequences. They can be used to search sequence data bases, evaluate similarity scores, and identify periodic structures based on local sequence similarity.

 

The FASTA program is a more sensitive derivative of the FASTP program, which can be used to search protein or DNA sequence data bases and can compare a protein sequence to a DNA sequence data base by translating the DNA data base as it is searched.

 

FASTA includes an additional step in the calculation of the initial pairwise similarity score that allows multiple regions of similarity to be joined to increase the score of related sequences.

 

The RDF2 program can be used to evaluate the significance of similarity scores using a shuffling method that preserves local sequence composition. The LFASTA program can display all the regions of local similarity between two sequences with scores greater than a threshold, using the same scoring parameters and a similar alignment algorithm; these local similarities can be displayed as a “graphic matrix” plot or as individual alignments.

 

In addition, these programs have been generalized to allow comparison of DNA or protein sequences based on a variety of alternative scoring matrices.

 

 

Alpha-bungarotoxin, CF405s

00002-100ug
EUR 161
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF680r

9-00003
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF680r

9-00003
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1
EUR 168
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012
EUR 383
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013
EUR 417
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014
EUR 466
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015
EUR 466
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016
EUR 523
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017
EUR 433
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020
EUR 314
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1
EUR 418
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021
EUR 344
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1
EUR 448
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022
EUR 491
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1
EUR 625
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023
EUR 281
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025
EUR 139
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026
EUR 494
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027
EUR 345
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028
EUR 428
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030
EUR 345
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033
EUR 345
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A

00042-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543

00043
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543

00043-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568

00044
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568

00044-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594

00045
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594

00045-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633

00046
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633

00046-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R

00047
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R

00047-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R

00048
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R

00048-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350

00049
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350

00049-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R

00050
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R

00050-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532

00051
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532

00051-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C

00052
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C

00052-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680

00053
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680

00053-T
EUR 147
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF430

00054
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF430

00054-T
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF440

00055
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF440

00055-T
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Cholera Toxin Subunit B, CF488A conjugate

00070
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF568 conjugate

00071
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF594 conjugate

00072
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF640R conjugate

00073
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF532 conjugate

00074
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF543 conjugate

00075
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF620R conjugate

00076
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF633 conjugate

00077
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF660R conjugate

00078
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF680R conjugate

00079
EUR 282
Description: Minimum order quantity: 1 unit of 100ug

Human Transferrin, CF488A conjugate

00081
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF543 conjugate

00082
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF568 conjugate

00083
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF594 conjugate

00084
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Leave A Comment