An integrated encyclopedia of DNA elements in the human genome.
The human genome encodes the blueprint of life, but the function of the vast majority of its nearly three billion bases is unknown. The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped regions of transcription, transcription factor association, chromatin structure and histone modification.
These data enabled us to assign biochemical functions for 80% of the genome, in particular outside of the well-studied protein-coding regions. Many discovered candidate regulatory elements are physically associated with one another and with expressed genes, providing new insights into the mechanisms of gene regulation.
The newly identified elements also show a statistical correspondence to sequence variants linked to human disease, and can thereby guide interpretation of this variation.
Overall, the project provides new insights into the organization and regulation of our genes and genome, and is an expansive resource of functional annotations for biomedical research.
Description: Precision is a key quality in genome editing and gene modification methods. Confirmation of successful gene editing is an important step in any set up, and could save both time and resources if experimental insufficiencies are caught early on. abm’s CRISPR Genomic Cleavage Detection Kit is designed as an easy, yet effective way to verify your genomic editing process.CRISPR-edited samples are used as a template in PCR reactions targeting your specific region of interest. A pair of primers flanking each sgRNA target site is required to detect genomic cleavage using the kit, and this can be ordered from abm (Cat. No. C336 - sgRNA PCR Primer Pair Design & Synthesis Service). The products are then denatured and re-annealed to produce mismatches within the double strand. Our detection enzyme is able to recognize such mismatches and cleaves the strands to produce band sizes that are easily distinguishable upon gel analysis. The ready-to-use CRISPR Genomic Cleavage Detection Kit conveniently contains all the necessary reagents required, including a set of control template and primers to ensure reliable results. The control template has an approximate size of 750bp after PCR amplification and of 500bp and 250bp after the enzymatic cleavage. With a short processing time, this ready-to-use assay will be a great addition to any genomic-editing toolbox.Kit Features:Ease of use with simple stepsRapid set-upStreamlined protocol suitable for high-throughput applications
A.I.I. (Acute Intestional Infections) Real-TM
Real Time PCR test for detection of Shigella Spp. E.coli, Salmonella spp.,
Campylobacter spp., Adenovirus F, Rotavirus A, Norovirus 2 genotype, Astrovirus
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.
Studies on transformation of Escherichia coli with plasmids.
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth in medium containing elevated levels of Mg2+, and incubation of the cells at 0 degrees C in a solution of Mn2+, Ca2+, Rb+ or K+, dimethyl sulfoxide, dithiothreitol, and hexamine cobalt (III). Transformation efficiency declines linearly with increasing plasmid size.
Relaxed and supercoiled plasmids transform with similar probabilities. Non-transforming DNAs compete consistent with mass. No significant variation is observed between competing DNAs of different source, complexity, length or form.
Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNAmolecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmids.
Transformation of intact yeast cells treated with alkali cations.
ntact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl.
The optimum incubation time was 1 h, and the optimum cell concentration was 5 x 10(7) cells per ml. The optimum concentration of Cs+ was 1.0 M. Transformation efficiency increased with increasing concentrations of plasmidDNA.
Polyethylene glycol was absolutely required. Heat pulse and various polyamines or basic proteins stimulated the uptake of plasmid DNA. Besides circular DNA, linear plasmid DNA was also taken up by Cs+-treated yeast cells, although the uptake efficiency was considerably reduced.
The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
Improved tools for biological sequence comparison.
We have developed three computer programs for comparisons of protein and DNA sequences. They can be used to search sequence data bases, evaluate similarity scores, and identify periodic structures based on local sequence similarity.
The FASTA program is a more sensitive derivative of the FASTP program, which can be used to search protein or DNAsequence data bases and can compare a protein sequence to a DNA sequence data base by translating the DNA data base as it is searched.
FASTA includes an additional step in the calculation of the initial pairwise similarity score that allows multiple regions of similarity to be joined to increase the score of related sequences.
The RDF2 program can be used to evaluate the significance of similarity scores using a shuffling method that preserves local sequence composition. The LFASTA program can display all the regions of local similarity between two sequences with scores greater than a threshold, using the same scoring parameters and a similar alignment algorithm; these local similarities can be displayed as a “graphic matrix” plot or as individual alignments.
In addition, these programs have been generalized to allow comparison of DNA or protein sequences based on a variety of alternative scoring matrices.
Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing.
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample.
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing.
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media.
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample.
Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA)
Cynthia
