Genomic Characterization defines Human Glioblastoma genes and core pathways

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Molecular portraits of human breast tumours.

Human breast tumours are diverse in their natural history and in their responsiveness to treatments. Variation in transcriptional programs accounts for much of the biological diversity of human cells and tumours. In each cell, signal transduction and regulatory systems transduce information from the cell’s identity to its environmental status, thereby controlling the level of expression of every gene in the genome. Here we have characterized variation in gene expression patterns in a set of 65 surgical specimens of human breast tumours from 42 different individuals, using complementary DNA microarrays representing 8,102 human genes.

 

These patterns provided a distinctive molecular portrait of each tumour. Twenty of the tumours were sampled twice, before and after a 16-week course of doxorubicin chemotherapy, and two tumours were paired with a lymph node metastasis from the same patient. Gene expression patterns in two tumour samples from the same individual were almost always more similar to each other than either was to any other sample.

 

Sets of co-expressed genes were identified for which variation in messenger RNA levels could be related to specific features of physiological variation. The tumours could be classified into subtypes distinguished by pervasive differences in their gene expression patterns.

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50/100 bp DNA Ladder (50 bp ~ 1,500 bp, with loading dye)

W109-100 Ask for price

Image Green? 100 bp DNA Marker

M1155-500
EUR 294

100 bp DNA Ladder

M1158-500
EUR 278

100 bp DNA Ladder

MD104-01
EUR 110

100 bp DNA Ladder

MD104-02
EUR 116

100 bp LadderDNA Marker HighConcentration

FYD005-500UL Ask for price

ACTGene? DNA Marker, 100 bp Ladder, 11 Fragments, 100 loads

ACT-IDMWD100
EUR 149

Star 100 bp DNA Ladder Express

BA01302
EUR 80
Description: High purity buffer for various PCR applications.

Wide range 100 bp DNA Ladder

M1192-500
EUR 278

50 bp Ladder DNA Marker

FYD004-500UL Ask for price

Ready-to-Use 100 BP DNA Ladder

31032
EUR 134
Description: Minimum order quantity: 1 unit of 1kit

100 bp DNA Ladder in TE buffer

31040
EUR 141
Description: Minimum order quantity: 1 unit of 300uL

PPAR-BP Blocking Peptide

AF6446-BP
EUR 195

CREB-BP Blocking Peptide

AF0139-BP
EUR 195

100 bp-10 Kb Wide Range DNA Logical Marker, Original Form

M103O-1
EUR 119.6

100 bp-10 Kb Wide Range DNA Logical Marker, Original Form

M103O-2
EUR 363.2

OneMark 100 DNA Ladder (ready-to-use, 100-3000 bp)

DM101-0100
EUR 63

Phospho-PPAR-BP (Thr1457) Blocking Peptide

AF3446-BP
EUR 195

100 bp-10 Kb Wide Range DNA Logical Marker, Ready-to-use

M103R-1
EUR 119.6

100 bp-10 Kb Wide Range DNA Logical Marker, Ready-to-use

M103R-2
EUR 363.2

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105
EUR 50

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105L Ask for price

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-105XL Ask for price

100-3000 bp DNA Rainbow Ladder PRODUCT (ready-to-use)

307-125
EUR 120

Genorise® Universal DNA Mini Kit 100

GR101076-100
EUR 357

BP-1-102

HY-100493
EUR 1345

Naproxen, BP grade

GP6640-25G
EUR 174

CREB-BP Antibody

33346-100ul
EUR 252

CREB-BP Antibody

33346-50ul
EUR 187

epsilon BP Antibody

abx018318-100ug
EUR 384

BP-1 Protein

20-abx263543
  • EUR 829.00
  • EUR 411.00
  • EUR 537.00

BP 554 maleate

B6351-10
EUR 268
Description: BP-554 maleate is a selective agonist of 5-HT1A receptor [1]. The 5-HT1A receptor is a G protein-coupled receptor for endogenous neurotransmitter serotonin (5-HT) and mediates inhibitory neurotransmission. BP-554 maleate is a selective 5-HT1A receptor agonist.

BP 554 maleate

B6351-50
EUR 973
Description: BP-554 maleate is a selective agonist of 5-HT1A receptor [1]. The 5-HT1A receptor is a G protein-coupled receptor for endogenous neurotransmitter serotonin (5-HT) and mediates inhibitory neurotransmission. BP-554 maleate is a selective 5-HT1A receptor agonist.

PPAR-BP Antibody

AF6446
EUR 304
Description: PPAR-BP Antibody detects endogenous levels of total PPAR-BP.

CREB- BP Antibody

ABF0139
EUR 438

PPAR- BP Antibody

ABF6446
EUR 438

CREB-BP Antibody

AF0139
EUR 304
Description: CREB-BP antibody detects endogenous levels of total CREB-BP.

20 bp TinyOrange DNA Ladder (105ng/µl)

309-005
EUR 108

20 bp TinyOrange DNA Ladder (105ng/µl)

309-025
EUR 323

100bp plus Ladder (no loading dye, 100-1500 bp)

306-005
EUR 50

100bp plus Ladder (no loading dye, 100-1500 bp)

306-025
EUR 120

Kanamycin Acid Sulfate BP

K004-25G
EUR 251

Kanamycin Acid Sulfate BP

K004-5G
EUR 82

Mouse BP ELISA Kit

EMB0690
EUR 521

AI-BP Polyclonal Antibody

ES1613-100ul
EUR 279
Description: A Rabbit Polyclonal antibody against AI-BP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

AI-BP Polyclonal Antibody

ES1613-50ul
EUR 207
Description: A Rabbit Polyclonal antibody against AI-BP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

Anserine BP ELISA Kit

EAB0690
EUR 521

Rabbit BP ELISA Kit

ERTB0690
EUR 521

Porcine BP ELISA Kit

EPB0690
EUR 521

Rat BP ELISA Kit

ERB0690
EUR 521

Human BP ELISA Kit

EHB0690
EUR 521

Canine BP ELISA Kit

ECB0690
EUR 521

Goat BP ELISA Kit

EGTB0690
EUR 521

Bovine BP ELISA Kit

EBB0690
EUR 521

Ran BP-1 antibody

22886-100ul
EUR 390

Anti-CREB-BP Antibody

A00205
EUR 397
Description: Rabbit Polyclonal CREB-BP Antibody. Validated in IF, IHC, WB and tested in Human.

AI-BP Polyclonal Antibody

ABP50614-003ml
EUR 158
Description: A polyclonal antibody for detection of AI-BP from Human, Mouse, Rat. This AI-BP antibody is for WB , IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AI-BP at AA range: 90-170

AI-BP Polyclonal Antibody

ABP50614-01ml
EUR 289
Description: A polyclonal antibody for detection of AI-BP from Human, Mouse, Rat. This AI-BP antibody is for WB , IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AI-BP at AA range: 90-170

AI-BP Polyclonal Antibody

ABP50614-02ml
EUR 414
Description: A polyclonal antibody for detection of AI-BP from Human, Mouse, Rat. This AI-BP antibody is for WB , IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human AI-BP at AA range: 90-170

Recombinant Human BP-1

7-04579 Ask for price

Recombinant Human BP-1

7-04580 Ask for price

Recombinant Human BP-1

7-04581 Ask for price

Anti-AI-BP antibody

STJ91512
EUR 197
Description: Rabbit polyclonal to AI-BP.

CREB-BP Conjugated Antibody

C33346
EUR 397

anti- MCM-BP antibody

FNab05064
EUR 505.25
Description: Antibody raised against MCM-BP

IL18 BP Recombinant Protein

96-467
EUR 490.25
Description: Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.

IL18 BP Recombinant Protein

96-469
EUR 542.75
Description: Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.

Anti-MCM-BP antibody

PAab05064
EUR 355

100 bp DNA Ladder Plus, ready-to-use, 50 ug, 0.5 ug per lane

Z6030001-1
EUR 109

100 bp DNA Ladder Plus, loading dye included, 50 ug, 0.5 ug per lane

Z6030001-2
EUR 102

100 bp DNA Ladder Plus, ready-to-use, 250 ug, 0.5 ug per lane

Z6030001-3
EUR 288

100 bp DNA Ladder Plus, loading dye included, 250 ug, 0.5 ug per lane

Z6030001-4
EUR 259

50bp DNA ladder (no loading dye) (50bp - 1000 bp)

300009
EUR 50

50bp DNA ladder (no loading dye) (50bp - 1000 bp)

300010
EUR 120

20 bp random library with flanking sequence; DNA aptamer

DAL-N-20
EUR 286

30 bp random library with flanking sequence; DNA aptamer

DAL-N-30
EUR 347

40 bp random library with flanking sequence; DNA aptamer

DAL-N-40
EUR 408

BP-1 Human Recombinant Protein

PROTQ92988
EUR 479
Description: BP-1 Human Recombinant (aa 103-168) expressed in E.coli, shows a 35 kDa band on SDS-PAGE.;The BP-1 is purified by proprietary chromatographic techniques.

Ran BP-17 Polyclonal Antibody

ES7204-100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Ran BP-17 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Ran BP-17 Polyclonal Antibody

ES7204-50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Ran BP-17 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

GAP1-InsP4 BP Polyclonal Antibody

ES2397-100ul
EUR 279
Description: A Rabbit Polyclonal antibody against GAP1-InsP4 BP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

GAP1-InsP4 BP Polyclonal Antibody

ES2397-50ul
EUR 207
Description: A Rabbit Polyclonal antibody against GAP1-InsP4 BP from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Ran BP-6 Polyclonal Antibody

ES3323-100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Ran BP-6 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, WB, ELISA

Ran BP-6 Polyclonal Antibody

ES3323-50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Ran BP-6 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, WB, ELISA

Lck BP-1 Polyclonal Antibody

ES5724-100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Lck BP-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Lck BP-1 Polyclonal Antibody

ES5724-50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Lck BP-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Guinea Pig BP ELISA Kit

EGB0690
EUR 521

 

Comprehensive genomic characterization defines human glioblastoma genes and core pathways.

Human cancer cells typically harbour multiple chromosomal aberrations, nucleotide substitutions and epigenetic modifications that drive malignant transformation. The Cancer Genome Atlas (TCGA) pilot project aims to assess the value of large-scale multi-dimensional analysis of these molecular characteristics in human cancer and to provide the data rapidly to the research community.
Here we report the interim integrative analysis of DNA copy number, gene expression and DNA methylation aberrations in 206 glioblastomas–the most common type of adult brain cancer–and nucleotide sequence aberrations in 91 of the 206 glioblastomas.
This analysis provides new insights into the roles of ERBB2, NF1 and TP53, uncovers frequent mutations of the phosphatidylinositol-3-OH kinase regulatory subunit gene PIK3R1, and provides a network view of the pathways altered in the development of glioblastoma.
Furthermore, integration of mutation, DNA methylation and clinical treatment data reveals a link between MGMT promoter methylation and a hypermutator phenotype consequent to mismatch repair deficiency in treated glioblastomas, an observation with potential clinical implications.
Together, these findings establish the feasibility and power of TCGA, demonstrating that it can rapidly expand knowledge of the molecular basis of cancer.

The sequence of the human genome.

A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion of the human genome was generated by the whole-genome shotgun sequencing method.
The 14.8-billion bp DNA sequence was generated over 9 months from 27,271,853 high-quality sequence reads (5.11-fold coverage of the genome) from both ends of plasmid clones made from the DNA of five individuals.
Two assembly strategies-a whole-genome assembly and a regional chromosome assembly-were used, each combining sequence data from Celera and the publicly funded genome effort.
The public data were shredded into 550-bp segments to create a 2.9-fold coverage of those genome regions that had been sequenced, without including biases inherent in the cloning and assembly procedure used by the publicly funded group.
This brought the effective coverage in the assemblies to eightfold, reducing the number and size of gaps in the final assembly over what would be obtained with 5.11-fold coverage.
The two assembly strategies yielded very similar results that largely agree with independent mapping data. The assemblies effectively cover the euchromatic regions of the human chromosomes.
More than 90% of the genome is in scaffold assemblies of 100,000 bp or more, and 25% of the genome is in scaffolds of 10 million bp or larger. Analysis of the genome sequence revealed 26,588 protein-encoding transcripts for which there was strong corroborating evidence and an additional approximately 12,000 computationally derived genes with mouse matches or other weak supporting evidence.
Although gene-dense clusters are obvious, almost half the genes are dispersed in low G+C sequence separated by large tracts of apparently noncoding sequence.
Only 1.1% of the genome is spanned by exons, whereas 24% is in introns, with 75% of the genome being intergenic DNA. Duplications of segmental blocks, ranging in size up to chromosomal lengths, are abundant throughout the genome and reveal a complex evolutionary history.
Comparative genomic analysis indicates vertebrate expansions of genes associated with neuronal function, with tissue-specific developmental regulation, and with the hemostasis and immune systems.
DNA sequence comparisons between the consensus sequence and publicly funded genome data provided locations of 2.1 million single-nucleotide polymorphisms (SNPs). A random pair of human haploid genomes differed at a rate of 1 bp per 1250 on average, but there was marked heterogeneity in the level of polymorphism across the genome.
Less than 1% of all SNPs resulted in variation in proteins, but the task of determining which SNPs have functional consequences remains an open challenge.

Alpha-bungarotoxin, CF680r

9-00003
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1
EUR 168
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012
EUR 383
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013
EUR 417
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014
EUR 466
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015
EUR 466
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016
EUR 523
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017
EUR 433
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020
EUR 314
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1
EUR 418
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021
EUR 344
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1
EUR 448
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022
EUR 491
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1
EUR 625
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023
EUR 281
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025
EUR 139
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026
EUR 494
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027
EUR 345
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028
EUR 428
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030
EUR 345
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033
EUR 345
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A

00042-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543

00043
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543

00043-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568

00044
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568

00044-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594

00045
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594

00045-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633

00046
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633

00046-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R

00047
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R

00047-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R

00048
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R

00048-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350

00049
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350

00049-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R

00050
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R

00050-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532

00051
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532

00051-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C

00052
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C

00052-T
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680

00053
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680

00053-T
EUR 147
Description: Minimum order quantity: 1 unit of 50U

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